Neonatal diarrhea is the main cause of morbidity and mortality in calves, and Rotavirus is the main viral etiology. The objective of the current study was to study the epidemiological role of Rotavirus infection in diarrheic neonatal cattle and buffaloes 'calves in Assiut Governorate, Egypt. From December 2015 to November 2019, a total number of 315 neonatal calves belonged to different localities of Assiut Governorate, Upper Egypt, were clinically examined. Fecal samples of investigated calves were subsequently collected and serologically tested to reveal-up Rotavirus infection by using latex agglutination test (LAT) and immunochrmatographic assay (ICA). The seropositive samples by LAT and ICA were 16.74% and 8.54%, respectively. The clinical findings of Rotavirus infection in enteric calves were pointed. The percentage of Rotavirus infection was 14.92% (44/295) of clinically diarrheic calves. However, 10% (2/20) of apparently healthy calves (n = 20) harbor Rotavirus in their feces suggesting carrier status. The high percentage of Rotavirus infection (20.13%) was obviously observed in 3 days-4 weeks old calves. There were no significance differences (P < 0.05) between the percentages of Rotavirus infection and sex, species (cattle and buffaloes) and breed (Native and Cross breed) of serologically tested calves. Moreover, there is no significant variations (p < 0.05) between calves under farmer’s hand and calves bred in farm in susceptibility to Rotavirus infection. Based on climatologic conditions of Assiut, seropositive cases were more prevalent (P < 0.001) in cold months (22.67%) than the warm and hot months (7.27%) in Assiut.

o investigate the role of NLRP3 inflammasome in cardiac aging, we evaluate here morphological and ultrastructural age-related changes of cardiac muscles fibers in wild-type and NLRP3-knockout mice, as well as studying the beneficial effect of melatonin therapy. The results clarified the beginning of the cardiac sarcopenia at the age of 12 months, with hypertrophy of cardiac myocytes, increased expression of β-MHC, appearance of small necrotic fibers, decline of cadiomyocyte number, destruction of mitochondrial cristae, appearance of small-sized residual bodies, and increased apoptotic nuclei ratio. These changes were progressed in the cardiac myocytes of 24 old mice, accompanied by excessive collagen deposition, higher expressions of IL-1α, IL-6, and TNFα, complete mitochondrial vacuolation and damage, myofibrils disorganization, multivesicular bodies formation, and nuclear fragmentation. Interestingly, cardiac myocytes of NLRP3^−/− mice showed less detectable age-related changes compared with WT mice. Oral melatonin therapy preserved the normal cardiomyocytes structure, restored cardiomyocytes number, and reduced β-MHC expression of cardiac hypertrophy. In addition, melatonin recovered mitochondrial architecture, reduced apoptosis and multivesicular bodies’ formation, and decreased expressions of β-MHC, IL-1α, and IL-6. Fewer cardiac sarcopenic changes and highly remarkable protective effects of melatonin treatment detected in aged cardiomyocytes of NLRP3^−/− mice compared with aged WT animals, confirming implication of the NLRP3 inflammasome in cardiac aging. Thus, NLRP3 suppression and …

This study was conducted in the kingdom of Saudi Arabia during the period from (August 2019 to August 2020) to investigate the sarcoptic mange infestation level in one humped camels (Camelus dromedarius), and to assess the effect of the age and season on the mange infestation, to assess the effect of the mange infestation on the appetite and body condition score (BCS) and to develop a good an applicable treatment protocol. 250 female camels were examined clinically and parasitologically, skin scraping revealed that Sarcoptes scabiei var. cameli mite was found in 28% of the examined animals, data analysis of the selected parameters showed that there was an effect for the age and season on the mange infestation, on the other hand there was no effect for the mange infestation on the appetite and BCS. Administration of Doramectin injection and Deltamethrin spray together founded to be the best treatment protocol for the sarcoptic mange infestation in camels, this finding indicates that using the acaricides alone is not enough for the disease eradication and must combined with treatment of the contaminated environment to prevent the reinfection from surrounding arears and achieve the highest cure level.

Toxoplasma gondii is intracellular protozoan parasite that are distributed worldwide and of major economic importance in the livestock industry especially sheep and goats. Sheep and goats are thought to be biological indicators of environmental contamination with T. gondii oocysts. In addition, in developing countries such as Saudi Arabia, where sheep and goat meat is commonly consumed, T. gondii infection in small ruminants may also affect public health risks. So that we estimate the prevalence of T. gondii infections in small ruminants, by using indirect enzyme-linked immunosorbent assay (ELISA) to detect the antibodies to assess the seroprevalence in 130 sheep and 130 goats from Dammam city. A total of 130 sheep were sampled, of which 35 (26.9%) were positive for T. gondii, out of 130 tested goats 31 (23.8%) were positive for T. gondii. Our study also recorded 27 out 52 from the aborted ewes (51.9%) and 22 out 45 from the aborted does (48.8%) were seropositive for anti-T. gondii antibody. Significant differences (p value < 0.0001) were observed among previously aborted females when evaluated as risk factors for T. gondii infection in both ewes and does. In addition, the results revealed that the age in sheep more than 3 years give 31.1% but in goat 23%. Significant differences (p value < 0.0001) were observed among previously ages, sex and the farming system, from the results of this study showed a broad distribution for protozoan parasite (Toxoplasma gondii) in examined sheep and goat flocks. By using ELISA Toxoplasma gondii antibody test kit that provides a rapid, simple, sensitive and specific method for diagnosis of Toxoplasmosis.

Keywords: Toxoplasma gondii; Sheep; Goats; Saudi Arabia; Elisa

Rotavirus is an enteritis causing pathogen in neonatal calves. A total number of 175 neonatal enteric calves were clinically examined. Their feces were sampled to rapidly serotested for Rotavirus detection. The clinical findings on Rotavirus diarrheic calves were anorexia, weakness, arched back with straining during defecation, variable degree of dehydration. Feces were pasty yellowish with and/or without flakes of clotted blood. Body temperature, respiratory and heart rates were varying. The collected fecal samples were serotested by Latex agglutination test (LAT) and Immunochromatographic assay (ICA). Results of LAT and ICA indicated that the positive samples of Rotavirus infection were 9.68% and 8.54%, respectively. It is concluded that Rotavirus infection plays an outstanding role in enteritis of neonatal calves of Assiut Governorate and control measures should be attained. LAT and ICA are effortlessly field serotests in screening of Rotavirus infection in diarrheic neonatal calves. Keywords: Rotavirus; Calf Diarrhea; LAT; ICA

The study aimed to detect the prevalence of camel filariasis in Upper Egypt, the effect of age, sex, season, locality and periodicity of sampling and treatment of infested cases and also determines the diagnostic technique for detection of the parasite. The study carried out on a total number of 350 camels (Camelus dromedarius) belonged to different Governorates in Upper Egypt including Assuit, Sohag, Asswan and El-wady El-gaded, by using the following methods for diagnosis of camel filariasis, wet blood film, thin blood film, thick blood film and concentration technique (Knott's technique). From the total number of examined camels (13 out of 350) camels were positive by blood film in percentage of 3.71%. The highest percentage of infection was recorded in El-Wady El-gaded Governorate (10.83%), hot season showed 4.96%, female more susceptible (7.79%) than male (2.56%), local breed more susceptible 5.9 …

The aim of this study is to identify the causative protozoan parasites infecting dogs in Egypt by discussing the clinical signs, hemato-biochemical, oxidative stress, and the pro-inflammatory markers alterations in Canine leishmaniosis (CanL). A total of 100 dogs of different breeds were collected from January to December 2020. The age of the dogs ranged between 3and 10 years. All the 100 dogs suffered from signs compatible with vector-borne diseases. Only one reported case was recorded for infection with CanL from 100 examined dogs (1% infection rate). The dog was 7 years old female German Sphered dog with significant weight loss, depression, pyrexia (temp= 40º), lameness on hind limbs, severe respiratory manifestations, and skin ecchymosis in the ventral abdomen, tail, and hind leg with lymphadenopathy. It had marked leukocytosis, neutrophilia, and monocytosis. Normocytic normochromic anemia and thrombocytopenia were also observed. The most important biochemical changes in the serum were mild hyperproteinemia, hypoalbuminemia, hyperglobulinemia, elevated liver and kidney function, elevation in C-reactive protein and malondialdehyde level and reduction in total antioxidant capacity.