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Role of Uterine Telocytes During Pregnancy

Research Abstract

Endometrial remolding and angiogenesis are critical events that occur during pregnancy in order to establish uteroplacental vascular
communication. This study investigated the role of uterine telocytes (TCs) in pregnancy. We analyzed the distribution of TCs and
morphological changes in the endometrium of the gravid rabbit uterus at different stages of pregnancy: after ovulation, pre-implantation (day
7), post-implantation (days 8 and 9), and mid-pregnancy (day 14) and late (days 21–28) pregnancy. TCs gradually increased with the
progression of pregnancy. They had distinctive telopodes (TPs) and podoms, with intranucleolar chromatin. The TCs established contact with
decidual cells, growing a glandular epithelium, blood vessels, and immune cells, such as lymphocytes, neutrophils, and macrophages. The
TCs underwent morphological changes at the post-implantation phase. They acquired thick and voluminous TPs, formed an extensive threedimensional
(3D) labyrinth at mid-pregnancy, and exhibited irregular-shaped nuclei and a dilated rough endoplasmic reticulum at late
pregnancy. They also acquired a convoluted contour-formed complex network. Scanning electron microscopy (SEM) showed an extensive 3D
network in the endometrium, forming a condensed sheath at late pregnancy. Transmission electron microscopy and SEM detected
fenestrated TPs, and TCs were identified by CD34 and vascular endothelial growth factor expression. TCs also expressed matrix
metalloproteinase-9 and transforming growth factor beta-1. Results suggested that TCs might play an essential role in maternal placenta
formation, especially decidualization, regulation of uterine gland development, and neovascularization of maternal uterine blood vessels.

Research Authors
Soha A. Soliman, Hanan H. Abd-Elhafeez,* , Alaa S. Abou-Elhamd , Basma Mohamed Kamel, Nada Abdellah and Fatma El-Zahraa A. Mustafa
Research Date
Research Journal
Microscopy and Microanalysis
Research Pages
283–302
Research Publisher
Oxford University Press
Research Vol
29
Research Year
2023

Monitoring antimicrobial residues in table eggs in Aswan governorate markets and their impact on egg quality and public health

Research Abstract

Abstract
Background: Organic egg is among the most common organic foods offered for sale in Egyptian markets in recent years, and consumers buy them at a higher price because they believe organic eggs are safer and have superior nutritional value than conventional eggs.
Aim: The present work aimed to monitor antimicrobial residues in brown table eggs, whether conventional or organic type, in Aswan governorate markets and assessed their physical and chemical quality and their public health hazards.
Methods: Brown table egg samples (n = 400 total) were randomly selected in the present study, in which they represented two equal groups (n = 200 each) including conventional eggs and organic eggs. Eggs were collected from different retail stores in the Aswan governorate, Egypt. Egg samples were subjected to thorough physical and chemical quality evaluation as well as an assessment of antimicrobial residues.
Results: The results reported that organic eggs were cleaner and had a better odor, less blood, and meat spots, but smaller with more shell cracks than conventional eggs. Chemical analysis of some nutrient contents in the egg yolk revealed significantly higher nutritive values of organic eggs than that of conventional ones as the organic eggs contain significantly higher levels of vitamin A and vitamin D/D3 and significantly lower values of cholesterol, calcium, magnesium, and zinc than those in conventional eggs. Disc diffusion assay has been used for monitoring antimicrobial residues in egg samples. The results have shown that all examined organic eggs were free from antimicrobial residues, while 12% and 8% of conventional egg yolk and white were positive for antimicrobial residues, respectively.
Conclusion: The study concludes the higher nutritive value of organic eggs compared with the conventional type because of their significantly higher contents of vitamins A and D and their significantly lower contents of cholesterol. Moreover, organic eggs were free from antimicrobial residues which maximizes their public health benefits.

Research Authors
Asem M. Zakaria, Enas Elmeligy, Abdulrahman Abdulkarim, Haitham H. Mohammed, Arafat Khalphallah, Mohammed Abdelhadi Ali, Marwa I. Khalifa
Research Date
Research Department
Research Journal
open veterinary journal
Research Pages
523-531
Research Publisher
https://www.openveterinaryjournal.com/
Research Rank
q2
Research Vol
13 (5)
Research Website
https://www.openveterinaryjournal.com/fulltext/100-1675951218.pdf?1684872816
Research Year
2023

Modulation of Immunity, Antioxidant Status, Performance, Blood Hematology, and Intestinal Histomorphometry in Response to Dietary Inclusion of Origanum majorana in Domestic Pigeons’ Diet

Research Abstract

This experiment was conducted to evaluate the effect of adding Origanum majorana (OM)
powder to domestic pigeon diets on growth performance, feeding and drinking behaviour, blood
hematology, blood biochemical parameters, blood inflammatory and oxidative markers, carcass
characteristics, the weights of lymphoid organs, and and intestinal cecal, and bursa of Fabricius
histology. A random distribution of fifty-four unsexed pigeon squabs (30 days old, average body
weight; 321 g  7.5) into three groups was done. The first group was fed the grower basal diet
without adding OM powder, while OM powder was added at levels of 0.5 and 1% to the basal
diets of the second and third groups, respectively. The changes in growth performance parameters
and feeding and drinking behavior under OM powder’s effect were insignificant. However, the
lymphoid organs (spleen and thymus) significantly increased in weight (p < 0.05) in the OM-fed
groups. Moreover, blood examination showed positive responses to OM powder in terms of blood
cell counts (RBCs andWBCs), and the values of hemoglobin, hematocrit, mean corpuscular volume,
lymphocyte numbers, levels of globulin, and glutathione peroxidase enzyme were significantly
increased. The numbers of heterophils, the ratio of heterophil to lymphocyte, malondialdehyde levels
were reduced (p < 0.05). Histomorphometry examination revealed increases in intestinal villi height,
cecal thickness, and bursal follicle area and number. These results indicated that adding OM powder
to the pigeon diet may improve their immunity, increase their antioxidant status, and correct some
hematological disorders.

Research Authors
Hala Y. Amer 1, Rasha I. M. Hassan 2, Fatma El-Zahraa A. Mustafa 3, Ramadan D. EL-Shoukary 4 , Ibrahim F. Rehan 5,6,*, František Zigo 7,* , Zuzana Lacková 7 and Walaa M. S. Gomaa 2,*
Research Date
Research Journal
Life
Research Pages
15
Research Year
2023

Field Trials to Evaluate Five Fasciolicides against Natural Liver Fluke Infection in Cattle and Sheep in Egypt

Research Abstract
Fasciola hepatica, a parasitic trematode, affects cattle and many mammals, including humans. The present study was
carried out in Assuit governate, Egypt, over one year from 2018 to 2019, to assess the prevalence o fascioliasis in cattle
and sheep. We clinically examined 835 animals (303 cattle and 532 sheep) from different private farms. We performed
the fecal examination through a direct smear and did a sedimentation technique. The results demonstrated that
fascioliasis was present in 20.8% of cattle and 17.1% of sheep, and the overall prevalence was 18.4%. After assessing
associated risk factors, there was a significant association only between sex and infection rate (P<0.05). Other assessed
risk factors (species, water, and feeding source) did not affect the infection level (P>0.05). By calculating the odds
ratio, the sex was considered as a risk factor as odds ratio (OR) =5.879, 95% confidence interval (CI) 3.699-9.449.
Categorized the animals into six groups. We treated each group with either albendazole, triclabendazole, superzole,
clorsulon, or rafoxanide. NC group received no treatment. Animals were subjected to clinical and laboratory
examination after the second dose's third and sixth week. The recovery percentage in animals treated with albendazole,
triclabendazole, and Superzole was 84%, while 84% and 96% in animals treated with clorsulon and rafoxanide,
respectively. All the drugs were effective (P<0.05); nonetheless, rafoxanide demonstrated the best recovery percentage
(area under curve =0.605 in cattle and =0.615 in sheep).

 

Research Authors
Walaa Mostafa1, Ahmed Abdel-Rady, M.F. El-Dakroury and Wael Felefel
Research Date
Research Department
Research File
Research Journal
International Journal of Veterinary Science
Research Member
Research Website
https://doi.org/10.47278/journal.ijvs/2022.160
Research Year
2023

Detection of Cryptosporidium parvum in calf feces using microscopical, serological, and molecular methods

Research Abstract
Cryptosporidiosis is a critical gastrointestinal disease in calves. This study examined 150 fecal samples of diarrheic calves collected from the eastern region of Saudi Arabia for detection of Cryptosporidium parvum using the Modified Ziehl-Neelsen (MZN) method, Enzyme-Linked Immunosorbent Assay (ELISA), and conventional Polymerase Chain Reaction (PCR). The performance of these methods was assessed using diagnostic accuracy tests. The present study identified C. parvum oocysts in fecal samples by modified Ziehl Neelsen 40/150; 26.66%, ELIZA 60/150; 40%, and PCR 78/150; 52% methods, respectively. The microscopic method revealed higher specificity 65.27% than the ELIZA 51.38%, while the ELIZA showed higher sensitivity 32.05% than MZN method 19.23%. However, MZN and ELIZA methods were unsatisfactory diagnostic tools compared with the PCR as the area under the curve values in Receiver Operator Characteristic (ROC) analysis were less than 0.6. Furthermore, using the kappa analysis test revealed no agreement between MZN and ELISA methods compared with PCR at P<0.05.

 

Research Authors
W. Felefel , A. Abd El-Rady , I. Abd El-Rahim, M.M. Elkamshishi and W. Mostafa6
Research Date
Research Department
Research File
Research Journal
Iraqi Journal of Veterinary Sciences
Research Member
Research Year
2023

Detection of Cryptosporidium parvum in calf feces using microscopical, serological, and molecular methods

Research Abstract
Cryptosporidiosis is a critical gastrointestinal disease in calves. This study examined 150 fecal samples of diarrheic calves collected from the eastern region of Saudi Arabia for detection of Cryptosporidium parvum using the Modified Ziehl-Neelsen (MZN) method, Enzyme-Linked Immunosorbent Assay (ELISA), and conventional Polymerase Chain Reaction (PCR). The performance of these methods was assessed using diagnostic accuracy tests. The present study identified C. parvum oocysts in fecal samples by modified Ziehl Neelsen 40/150; 26.66%, ELIZA 60/150; 40%, and PCR 78/150; 52% methods, respectively. The microscopic method revealed higher specificity 65.27% than the ELIZA 51.38%, while the ELIZA showed higher sensitivity 32.05% than MZN method 19.23%. However, MZN and ELIZA methods were unsatisfactory diagnostic tools compared with the PCR as the area under the curve values in Receiver Operator Characteristic (ROC) analysis were less than 0.6. Furthermore, using the kappa analysis test revealed no agreement between MZN and ELISA methods compared with PCR at P<0.05.

 

Research Authors
W. Felefel , A. Abd El-Rady , I. Abd El-Rahim , M.M. Elkamshishi and W. Mostafa
Research Date
Research Department
Research File
Research Journal
Iraqi Journal of Veterinary Sciences, Vol. 37, No. 2, 2023 (383-389)
Research Member
Research Publisher
Iraqi Journal of Veterinary Sciences, Vol. 37, No. 2, 2023 (383-389)
Research Vol
Vol. 37, No. 2, 2023 (383-389)
Research Website
(http://creativecommons.org/licenses/by/4.0/).

Some Epidemiological Studies on Theileria annulata Infection in Egypt

Research Abstract

The present study was carried out to investigate the epidemiological and clinical status of bovine Theileriosis in Aswan Governorate. During a 2-year study, 265 cattle were clinically suspected upon careful clinical examination as Theileria infected animals. Conventional diagnosis based on blood and lymph smears examinations showed that, the prevalence of Tropical Theileriosis in cattle in Aswan Governorate was 56 (21.13%). Giemsa stained blood smears showed presence of macro-schizont inside lymphocyte (Koch’s blue bodies), micro-schizonts inside lymphocyte, raptured schizont and intraerythrocytic stages of Theileria annulata piroplasms inside RBCs. Polymerase chain reactions of T. annulata merozoite-piroplasm surface antigen Targeting gene: (Tams1) revealed positive 29 (58%) animals confirmed by visualization of specific bands at 768 bP. Positive results could be detected in suspected cattle that showed positive or negative blood smear results that proved the high sensitivity of PCR test compared with the conventional method for diagnosis of bovine tropical Theileriosis. PCR proved a highly sensitive and accurate method for diagnosis of bovine tropical Theileriosis, especially in detection of blood and lymph smears negative cases.

Keywords | Epidemiology, Theileria annulata, Bovine, Diagnosis, PCR

Research Authors
Ahmed Abdel-Rady, Mohamed Karmi , Menna_allah Youssef, Aml M. Abdel-Ra’ouf, Bahaaa Madkour
Research Date
Research Department
Research Journal
Research Journal of Veterinary Practitioners
Research Member
Research Pages
7-12
Research Publisher
Research Journal of Veterinary Practitioners
Research Vol
11
Research Website
DOI | http://dx.doi.org/10.17582/journal.rjvp/2023/11.1.7.12
Research Year
2023

New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study

Research Abstract

Fertility in birds is dependent on their ability to store adequate populations of viable sperm for extended durations in sperm storage tubules (SSTs). The exact mechanisms by which sperm enter, reside, and egress from the SSTs are still controversial. Sharkasi chicken sperm showed a high tendency to agglutinate, forming motile thread-like bundles comprising many cells. Since it is difficult to observe sperm motility and behavior inside the opaque oviduct, we employed a microfluidic device with a microchannel cross-section resembling close to that of sperm glands allowing for the study of sperm agglutination and motility behavior. This study discusses how sperm bundles are formed, how they move, and what role they may have in extending sperm residency inside the SSTs. We investigated sperm velocity and rheotaxis behavior when a fluid flow was generated inside a microfluidic channel by hydrostatic pressure (flow velocity = 33 µm/s). Spermatozoa tended to swim against the flow (positive rheotaxis) and sperm bundles had significantly lower velocity compared to lonesome sperm. Sperm bundles were observed to swim in a spiral-like motion and to grow in length and thickness as more lonesome sperm are recruited. Sperm bundles were observed approaching and adhering to the sidewalls of the microfluidic channels to avoid being swept with fluid flow velocity > 33 µm/s. Scanning and transmission electron microscopy revealed that sperm bundles were supported by a copious dense substance. The findings show the distinct motility of Sharkasi chicken sperm, as well as sperm's capacity to agglutinate and form motile bundles, which provides a better understanding of long-term sperm storage in the SSTs.

Research Authors
Taymour M. El-Sherry, Hanan H. Abd-Elhafeez & M. A. M. Sayed
Research Date
Research Department
Research Journal
Scientific Reports
Research Pages
13003
Research Publisher
Springer Nature
Research Rank
Q1
Research Vol
12
Research Website
https://doi.org/10.1038/s41598-022-17037-x
Research Year
2022

New insights into the combined toxicity of aflatoxin B1 and fumonisin B1 in HepG2 cells using Seahorse respirometry analysis and RNA transcriptome sequencing

Research Abstract

Aflatoxin B1 (AFB1) and fumonisin B1 (FB1) are widely (co-)detected in food and known for their hepatotoxicity in humans. Still, their combined toxicity needs to be investigated, especially the impact on mitochondria. In our previous work, we examined the effect of short-term exposure to different doses of AFB1, FB1, and their binary mixture (MIX) on the bioenergetic status of HepG2 cells, a well-recognized in vitro model system for studying liver cell function. In the current work, we further investigated the (combined) effect of AFB1 and FB1 on the mitochondrial and glycolytic activity of HepG2 cells using Seahorse respirometry analysis and RNA transcriptome sequencing. The results showed that the co-exposure, especially at high doses, is more toxic due to a more inhibition of all parameters of mitochondrial respiration. However, FB1 contributes more to the MIX effects than AFB1. RNA transcriptome sequencing showed that the p53 signaling pathway, a major orchestrator of mitochondrial apoptosis, was differentially expressed. Moreover, the co-exposure significantly downregulated the genes encoding for Complexes I, II, III, and IV, representing the onset of the suppressed mitochondrial respiration in HepG2 cells.

Research Authors
Xiangrong Chen , Mohamed Fathi Abdallah, Charlotte Grootaert , Filip Van Nieuwerburgh and Andreja Rajkovic
Research Date
Research Department
Research Journal
Environment International
Research Member
Research Pages
107945
Research Publisher
Elsevier
Research Website
https://www.sciencedirect.com/science/article/pii/S0160412023002180?via%3Dihub
Research Year
2023
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