An efficient micropropagation protocol for Koelreuteria bipinnata, an ornamental tree, from mature and juvenile phase tissues was described. Nodal explants from one-year-old branches of a field-grown mature tree (mature phase) and from in vitro growing seedlings (juvenile phase) were used. The nodal explants from mature explants cultured on Murashig& Skoog (MS) medium supplemented with or without growth regulators did not show any axillary shoot development. However, 6-benzylaminopurine (BAP) at 1.5 mg/l induced axillary shoots from all juvenile explants (100%). It also produced the highest number of shoots (2.5 shoots per explant), the highest rooting percentage (100%), the maximum number of roots (2.80 roots/shoot) and the longest roots (5.87 cm) when it was added as a supplement to the half-strength MS medium culture that included 15 g/l sucrose. Plantlets were successfully acclimatized and transferred to the field with 60% survival rate.