Two new mite species of the genus Caloglyphus Berlese, 1923 (Acari: Acaridae) are described and illustrated based on deutonymphal stages collected from mango orchards in Assiut, Upper Egypt. The new species, C. mangiferus sp. nov. and C. similares sp. nov., were extracted from soil samples under the mango trees. The new species are characterized by having a pair of short peduncles globosely setae situated dorsosublaterally on the idiosoma.