Experiments were carried out during two consecutive 2017/2018 and 2018/2019 seasons to measure the influence of various sources of nitrogen fertilizers on enhancing growth and fruiting of Williams banana plants grown under Qena Governorate, Egypt. Applying 25% of the recommended nitrogen dose along with 25 to 50% organic-N enriched with EM as a bio-form at 50 or 25% were effective in enhancing all growth aspects, production and fruit quality compared to the control. Using combination of three forms of nitrogen were more favorable than using mineral-N alone.Therefore, it could be concluded that using three forms of nitrogen fertilizers get high yield with good fruit quality of Williams bananas.  In addition, it reduces the cost of production and environmental pollution, due to excess of chemical fertilizers..