In this study, a 30-day incubation experiment was performed to investigate the interactive effects of soil moisture content and two types of organic manure (animal manure: M and wheat straw: WS) on organic C mineralization and the degradation of PAH compounds. Specifically, washed sandy soil sample free from PAHs was treated with combined standard solution containing six different PAHs; pyrene (Pyr), fluoranthene (Flt), benzo[a]pyrene (BaP), benzo[g,h,i]perylene (BghiP), benzo[k]fluoranthene (BkF), and indeno[123-cd]pyrene (IP). The soil samples treated with PAHs were amended with M or WS and then, the soil samples were incubated and subjected to two levels of moisture content (50% and 100% field capacity, FC). The results indicate that CO2–C rates were the highest at day 1, but they tended to be decreased sharply when incubation time increased. The results showed that the higher rate of CO2-C efflux rate and cumulative were observed in M and WS treatments at 100% FC. Applying organic amendments at 50% FC increased the total cumulative CO2-C from 21.6 mg kg−1 to 228 mg kg−1 for M and to 216 mg kg−1 for WS. Meanwhile, applying organic amendments at 50% FC increased the total cumulative CO2-C from 30 mg kg−1 to 381 mg kg−1 for M and to 492 mg kg−1 for WS. The highest increases at 100% FC could be explained by the optimum water content at field capacity. PAHs concentrations decreased significantly in the presence of organic amendments in relation to enhance CO2-C efflux (soil respiration) and to decrease soil pH. It could be concluded that applying organic amendments might be a useful technique to remediate soil PAHs through mineralization.