Thepresentstudyinvestigatedtheinflu-enceofexposingquaileggstolow-dosegammaradiation(GR)andinovofeedingwith2sourcesofamixtureoftraceelements(Zn,Fe,andCu),includingsulfate(TES)andloadedwithmontmorillonite(TEM),onembryonicdevelopmentactivitiesandprehatchquality.Atotalof960eggsontheseventhdayofincubationwererandomlydividedinto6groups(160eggs/group)with4replicateof40eggsineach.A3£2factorialarrangementexperimentwasperformedandincluded3sourcesinovofeedingwithamixtureoftraceelements(Zn,Fe,andCu),including0mg/egg,50mgTES/egg,and50mgTEM/eggwitheggirradiationusing0and0.2GyfromGR.Eggsinjectedwith50mgTEM/eggandexposedto0.2GyfromGR(TEM/GR)wassignificantly(P≤0.05and0.01)higherinhatchability,hatchbodyweight,andrelativeorganweight(liver,gizzard,proventriculus,heart,andintes-tine).Theobtainedresultsindicatedsignificant(P≤0.05)decreasedintheserumconcentrationofmalondialdehyde(MDA)inTEM/GRgroup.Therewassignificant(P≤0.05)increasedofcatalase(CAT)activityandthe concentrationsofgrowthhormone(GH)andinsulin-likegrowthfactor-1(IGF-1)inTEM/GRgroup;however;totalantioxidantcapacity(T-AOC)wassignificant(P≤0.05)increasedinCT/GRgroup.SerumconcentrationsofimmunoglobulinM(IgM)(P≤0.05)andtumornecrosisfactor-alpha(TNF-a)wereincreasedintheTEM/CRgroup;theconcentrationoftransforminggrowthfactorbeta(TGF-b)significant(P≤0.05)increasedintheTEM/GRgroup;andinterleukins(IL6andIL10)showednosignificantdifferencesamongthegroups.Ourresultsshowedincreaseinthyroxineandmyostatincon-centrationswithTES/CRandCT/GRofourstudygroups,respectively.TherelativemRNAexpressionlevelsoftheGH,IGF-1,andFascellsurfacedeathreceptor(FAS)genesweresignificantly(P≤0.05and0.01)upre-gulatedinthelivertissueoftheTEM/GRgroupcom-paredwiththeothergroups.Inconclusion,TEM/GRwasthebesttreatmentforimprovingprehatchquality,increasingserumantioxidantenzymeactivities,andpro-motingtheexpressionofgrowthandimmunegenesinfer-tilizedquaileggs.