Flutamide (Flu) is a chemotherapeutic drug, used for the treatment of prostate cancer. It functions by
interfering DNA in fast growing cells and preventing them from reproducing. The present work is focused
on the interaction of Flu with single and double stranded DNA at different temperatures and at
physiological pH 7.4 (human blood pH). Cyclic voltammetry, square wave voltammetry and UV–visible
spectroscopy were used to analyze the interaction of Flu with DNA. The voltammetric results indicate
Flu gets intercalated between dsDNA bases and the strength of interaction is independent on the ionic
strength. The hyperchromic effect in absorption spectra of Flu-dsDNA complex affirmed the intercalative
mode of binding between Flu and dsDNA. Comparison of the mode interaction of Flu with dsDNA and
ssDNA was discussed. The binding constants, stoichiometric coefficients and thermodynamic parameters
of Flu-dsDNA and Flu-ssDNA complexes were evaluated. The association between Flu and dsDNA is
maximum at 308 K which depicts the most stable complexes are formed at near human body tempera-ture. The decrease in the peak current of Flu resulting from its interaction with DNA was employed for
determination of dsDNA and ssDNA concentration. The detection limits of dsDNA and ssDNA were found
to be 4.2710
7
M and 1.8710
7
M, respectively