The aim of this study is to evaluate the effect of different concentrations of citral on
glutathione (GSH), nitric oxide (NO), lipid peroxide (LPO) and vitamin C. Also, the protective effect of N.
sativa was studied. Fertilized eggs of the chick Gallus domesticus were divided into control or experimental
groups which received three different concentrations of citral (50, 100 and 200 μM), N. sativa extract (5 μl) or
a combination of N. sativa extract with citral. Citral and N. sativa groups decreased GSH & vitamin C and
NO, while increased LPO. Co-treatment with citral and N. sativa increased GSH & vitamin C and decreased
LPO levels. Citral induced oxidative stress by inhibiting RA synthesis. The limited mitigative properties of N.
sativa are attributed to either its antineoplastic properties, the high levels of oxidative stress provoked by
citral and above its antioxidative capacity or the low administered dose.