exopolysaccharides (ePSs) are novel functional additives for low-fat yogurt. Pharmaceutical, medical, and food industries are using more lAB-based ePSs. in this study, leuconostoc spp. was used to produce ninth bacterial ePSs in a modified molasses medium. Production of ePSs was concentration-dependent on all stains and the highest yield was obtained from the S3 strain (55.23 g/l), followed by S6 (49.95 g/l), S8 (45.68 g/l), and S7 (44.23) , respectively. HPlC and FtiR analysis showed that all purified ePSs from leuconostoc citreum (S3) and leuconstoc holzaapfelii (S8) were related to exopolysaccharide glucan.  Anticancer activity of all ePSs samples (ePSs1-9) against Caco-2 cells and normal MCR-5 cells were investigated using Mtt assay. the results revealed that Caco-2 cells were more sensitive than the normal MCR-5 cells. the highest anticancer activity against Caco-2 cancer cells was recorded for ePS8 (iC50 = 22.94 µg/ml, Si=3.73), followed by ePS3 (iC50 = 36.15 µg/ml, Si=8.72), ePS1 (iC50 = 50.01 µg/ml, Si=3.73), and ePS4 (iC50 = 94.90 µg/ml, Si=3.26), respectively. the lowest cytotoxicity was recorded for ePS5 (iC50 = 130.5 µg/ml). the most active ePSs (ePS3 and ePS8) were used as fat replacements and stabilizers in low-fat set yogurt at non-toxic concentrations (0.4, 0.8, and 1.2%). ePS3 and ePS8 improved the low-fat yogurt's organoleptic and rheological properties. ePS8 had the highest water holding capacity (77.26%), viscosity (3660 CP), and lowest syneresis (22.95%) and whey off (0.6 ml). low-fat set yogurt enhanced with ePS3 and ePS8 recorded the highest sensory evaluation values with overall acceptability, especially ePS3b, ePS3c, ePS8c, and ePS8b; the total score point of 97.50, 97.43, 96.51, and 96.36, respectively  in fresh age compared to control yogurt (92.64). in conclusion, leuconostoc ePSs, especially ePS8, can be explored for anti-cancer effects on Caco-2 colorectal cancer cells. it could also improve the rheological and organoleptic qualities of low-fat set yogurt.