The endophytic fungus Paecilomyces sp. (AUMC 15510) was isolated from healthy stem samples of the
Egyptian medicinal plant Cornulaca monacantha. We used GC–MS and HPLC analysis to identify the
bioactive constituents of ethyl acetate crude extract of Paecilomyces sp. (PsEAE). Six human microbial
pathogens have been selected to evaluate the antimicrobial activity of PsEAE. Our data showed
that the extract has significant antimicrobial activity against all tested pathogens. However, the
best inhibitory effect was observed against Bacillus subtilis ATCC 6633 and Pseudomonas aeruginosa
ATCC 90274 with a minimum inhibitory concentration (MIC) of 3.9 μg/ml and minimum bactericidal
concentration (MBC) of 15.6 μg/ml, for both pathogens. Also, PsEAE exerts a significant inhibition on
the biofilm formation of the previously mentioned pathogenic strains. In addition, we evaluated the
wound healing efficiency of PsEAE on earthworms (Lumbricus castaneus) as a feasible and plausible
model that mimics human skin. Interestingly, PsEAE exhibited a promising wound healing activity and
enhanced wound closure. In conclusion, Paecilomyces sp. (AUMC 15510) could be a sustainable source
of antimicrobial agents and a potential therapeutic target for wound management.