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Comparison of an indirect format ELISA on modified graphite and polystyrene surfaces against Triazines.

Research Authors
D. Fouad, I. Sound, H. Holthues, U.P. Fukumura, A.M. Hammam,S.A. Ibrahim, and W. Baumann
Research Abstract

An indirect format enzyme-linked immuno-sorbent assay (ELISA) on graphite rods (∅ 0.8 mm x 20 mm) and, for comparison, on microtiter plates has been developed against terbuthylazine. For this purpose, a series of 2-aminoalkyl-4-chloro-6-terbuthyl-s-triazine-2,6-diamine ELISA haptens with alkyl spacer lengths of 2, 4, 6, and 8 CH2 groups has been synthesized. The graphite rods or the microtiter plates were covered by a polymerized glutaraldehyde network, and the ELISA haptens have been coupled by imino coupling to the free aldehyde groups of that network. ε-Aminocaproic acid has been used as an agent to block unspecific binding sites. The ELISA was run in a competitive mode, where the immobilized ELISA hapten and the solute terbuthylazine as a target analyte compete for the solute antibody.

Research Department
Research Journal
Z. Naturforsch
Research Rank
1
Research Vol
Vol 61 No.3- 4
Research Year
2006
Research Pages
pp. 295-301