A new method for synthesizing 4amino3methyl1phenyl1H5substituted thieno[2,3c]pyrazole was
reported. The substituted groups at position 5 include carbonitrile, carboxamide, Nphenyl carboxamide,
and benzoyl groups. The newly synthesized compounds and their derivatives were characterized by elemental
analysis and spectroscopy (IR, 1H NMR, and mass spectra). Furthermore, some of these synthesized com
pounds were screened against various pathogenic bacterial and fungal strains. The results demonstrate that
most of the synthesized compounds possess a significant antibacterial activity against grampositive and
gramnegative bacteria. In addition, most of these compounds showed a remarkable antifungal activity. On
the other hand, some of the synthesized compounds possess high antiinflammatory activity, which was dem
onstrated using the carrageenaninduced rat paw edema assay.