The effect of the electrical currents 3, 5 and 10 mA on glycogen, nonglycogen polysaccharides and total protein contents of hind limb regeneration in a premetamorphic stage (stage 56) and a metamorphic stage (stage 58) of the Egyptian toad, Bufo regularis Reuss was studied after amputation at the distal third of the thigh and mid shank levels. The amount of glycogen, mucopolysaccharides and total protein in the various tissues of limb regenerates was increased after 15 days of amputation that those of after five days both in control and experimental cases. It was suggested that the increase in polysaccharides and proteins are due to the restoration of aerobic cellular respiration and the relatively slow rate of building up new cells. Stimulation of the amputated limbs at the shank level was slightly more obvious than that at the thigh level.