The effect of intraperitoneal injection of growth hormone on hind limb regeneration was studied in two metamorphic stages of tadpoles of the Egyptian toad, Bufo regularis Reuss, after amputation at the mid shank level. Growth hormone enhanced limb regeneration in stage 56, where 77.8% of the cases regenerated toes ranging from five to one compared with 72.33% in the control group. Also, the differential effect of growth hormone on the number of toes was obvious in the treated animals, where 4.4% and 57.8% of the cases regenerated five and four toes respectively compared with 2.13% and 40.4% in the control group. In the metamorphic stage (stage 58), the effect of growth hormone was also obvious, where 75% of the treated cases restored part of the foot compared with 55.7% of the cases in the control group. Histological observations of the treated limbs revealed thick epithelial covering and complete skin faster than that of the control animals. Considerable amounts of cartilage were a histological characteristic of the treated limbs. It may be suggested that the limited enhancing effect of growth hormone on limb regeneration may be due to its acceleration of wound healing and shortening the period during which regeneration-stimulating factors pass from the epidermis to the underlying stump tissues.