Hydrogen gas (H₂) is an environmentally benign and sustainable energy fuel. Because of its high energy content, hydrogen presents a viable clean energy source alternative to fossil fuels and is regarded as a one of the most promising energy sources. In the current investigation, CuWO₄ was applied, for the first time, as an efficient catalyst for the green generation of H₂ from the hydrolysis of NaBH₄. CuWO₄ was fabricated via a co-precipitation assisted hydrothermal method. The synthesized catalyst was characterized by XRD, XPS, VSM, FTIR, SEM, TEM, and nitrogen sorption analyses. XRD and XPS analyses confirmed the successful formation of CuWO₄. It was estimated that, values of hydrogen generation rate (HGR) 818, 1250, 2467, and 2920 ml min⁻¹ g⁻¹ were respectively obtained at reaction temperatures of 28, 35, 40, and 45 °C. According to the pseudo-first-order equation, CuWO₄ have an estimated apparent activation energy of 59.2 kJ mol⁻¹. Thermodynamic parameters like ∆H^#, ∆S^#, and ∆G^# were also calculated. The antibacterial efficacy of CuWO₄ was evaluated against four Gram positive pathogenic strains; Bacillus subtilis, Bacillus cereus, Staphylococcus aureus and Micrococcus luteus with concentration range 0–150 µg ml⁻¹ comparing with chloramphenicol (CHL) antibacterial agent. Minimum inhibitory concentration (MIC) was determined for CuWO₄ and CHL for the four strains. CuWO₄ clear promising antibacterial properties with growth inhibition (%) of 82.79 (49.9 CHL) %, 73.56 (67.89 CHL) %, 61.38 (58.18 CHL) %, and 50.47 (43.4 CHL) % at 150 µg ml⁻¹ of B. subtilis, S. aureus, B. cereus, and Micrococcus luteus, respectively.