Soils are rich with various precious fungi that produce significant metabolites which could solve many medical issues of human life. Kojic acid represents a significant medical metabolite produced by fungi and used as anti-browning, anti-cancer, and anti-diabetic agent in medical products. In this study, a total of 31 species plus 3 varieties belonging to 14 fungal genera were recovered as rhizospheric fungi from the roots area of 30 samples of 27 different plants. The most common isolated genus was Aspergillus however; the most common isolated species were Aspergillus parasiticus, and A. flavus. Fifty two fungal isolates were tested for their ability to produce kojic acid, only 25 fungal isolates have the ability to produce kojic acid with various degrees and 27 isolates couldn’t produce it. Aspergillus flavus var. columnaris Af-19, A. parasiticus Ap-48, A. flavus var. columnaris Af-7, A. flavus Af-52, and A. flavus Af-40 were the high producers of kojic acid giving (g/l) 42.68, 39.96, 39.85, 39.79, and 38.92 kojic acid, respectively. The percent of kojic acid yields based on consumed sugar by these five fungal isolates were 48.60, 44.84, 43.52, 45.00 and 43.62%, respectively. From the obtained data, it could be assumed that rhizospheric fungi represent a promising producers of kojic acid as significant fungal metabolite.