The present study aimed to investigate changes in the protein contents using SDS-PAGE, also using the random amplified polymorphic DNA (RAPD-PCR) assay to identify changes in DNA concentra- tion in the embryos of Egyptian toad Bufo regularis. Our experiment started when tadpoles began to feed. The adapted embryos were divided into 3 large tanks of 200 embryos each, collections of samples started from feeding age every three days. Cluster method was used to indicate the dis- tinct distance between the band patterns of different stages. An increase occurred in the concen- tration of protein fractions in these tadpoles upon metamorphosis and that when metamorphosis was completed. DNA concentration exhibited an evident low values at the premetamorphic stage 44 (105.85 ± 0.519 ng/µl) compared with the other more advanced developmental stages 55 and 56 (204.056 ± 0.651 ng/µl and 234.55 ± 2.325 ng/µl respectively). In conclusion, acquire informa- tion about the changes in the molecular content of Bufo regularis during metamorphosis has been estimated.