Linguatula serrata is a neglected parasitic zoonosis with less research recorded throughout the globe on the physiological and immunological alteration of dogs infested by these zoonotic parasites. This study aimed to evaluate the effects of L. serrata infestation on the dogs after experimental infestation with the nymphs by determining oxidative stress levels and immunological genes of cellular immunity. Larvae of L. serrata were collected from the infested goat mesenteric lymph nodes by cutting it, and the nymphs were liberated; these nymphs were mixed with saline at 37–40 °C for experimental infestation of dogs and observed infestation to puppies using viable nymphal stages (movable, not traumatized or dead). Blood and sera samples were sampled from the dog after 6 months of infestation. The nasal mucosa and its mucus were collected from the infested dogs as well as negative controls for evaluation of …