Abstract: Bean seedlings 7-days old were left to grow for 10 days in hydroponic cultures containing 0, 5, 15 ppm Nickel as NiCl2 Calcium was added (1 mM or 5 mM) in combination with Nickel. Growth criteria, mineral contents, nickel distribution and metal-binding proteins were investigated. The fresh and dry weights of roots and shoots were sharply reduced especially at the higher dose (15 ppm Ni+2). The content of some elements (K, Na , Ca, Mg and Mn) of roots and shoots were variably affected in presence or absence of calcium. Nickel was accumulated in roots more than in shoots. Fractionation of roots and shoot of bean plants revealed that the accumulation of Nickel was in the insoluble form (cell wall), while the remainder (the soluble form) was bound to proteins in the form of metalloproteins. Calcium reduced the toxic effect of nickel especially at the low dose (5 ppm). It was found that calcium reduced the uptake of nickel by roots and enhanced the synthesis of proteins, which form metal-binding proteins.