Purpose: To study the adverse impacts of ultraviolet radiation-A (UVA 320–400 nm) on some
hematological and biochemical parameters of Bufo regularis was considered.
Materials and methods: Samples were classified into four groups: (i) Control; (ii) ultraviolet radiation
(UVR)-treated group (for 3 days/for 15 min/day); (iii) UVR-treated group (for 3 days/for 30 min/day);
and (iv) (for 3 days/for 60 min/day). The destructive effects of UVA radiation was evaluated by red
blood cells (RBC) count, hemoglobin content (Hb), hematocrite (Ht), erythrocytic indices, white
blood cells (WBC) count, total protein, glucose, aspartic amino transferase (AST), alanine amino
transferase (ALT), alkaline phosphatase (ALP), lactate dehyderogenase (LDH), glucose-6-phosphate
dehyderogenase (G6PDH) and total bilribuin.
Results: No mortality was observed. However, some physiological effects after the exposure to UVA
were reported. The UVA-induced malformations recorded in the red blood cells included crenated
cells (Cr), Acanthocytes (Ac), tear drop-like cells (Tr) and sickle cells (Sk).
Conclusion: The present study revealed the exposure to UVA from 15–60 min/day for three days
could promote several biochemical and physiological disturbances as well as some changes in RBC.