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Molecular Characterization of Mucor circinelloides and Rhizopus stolonifer Strains Isolated from Some Saudi Fruits

Research Authors
Gherbawy Y. A. and Nemmat A. Hussein
Research Abstract

Thirteen strains of Mucor circinelloides and nine strains of Rhizopus stolonifer were collected from different Saudi
fruits. Also, two strains of M. circinelloides and one strain of R. stolonifer isolated from soil were used in this study
to analyze the genetic diversity among these 25 strains. The dendrogram constructed from random amplified
polymorphic DNA–polymerase chain reaction results using two primers (V6 and M13) showed no correlation
between clustering system and sources of isolation for M. circinelloides strains, while Rhizopus strains clustered
according to the sources of isolation. The phylogenetic tree based on the unweighted pair group method with
arithmetic average of internal transcribed spacer (ITS) rDNA sequence revealed the variation of the ITS region
among Mucor strains as well as Rhizopus strains

Research Journal
Foodborne Pathogens and Disease
Research Publisher
NULL
Research Rank
1
Research Vol
7(2)
Research Website
NULL
Research Year
2010
Research Pages
137-142