Recently, the oxidative stress and immunotoxicity biomarkers have been extensively used in embryotoxicity using fish embryos
as promising models especially after exposure to chemical-like environmental estrogens. Bisphenol-A (BPA) is an estrogenic
endocrine disruptor and is ubiquitous in the aquatic environment. Larvae of Labeo rohita were exposed to low concentrations of
BPA(10, 100, 1000 μg/l) for 21 days. Innate immune system, antioxidants parameters, and developmental alterations were used
as biomarkers. Exposure to BPA caused developmental abnormalities including un-inflated swim bladder, delayed yolk sac
absorption, spinal curvature, and edema of pericardium. Lipid peroxidation increased and activity of catalase (p < 0.05), superoxide
dismutase (p < 0.05), and glutathione peroxidase (p < 0.01) decreased after exposure to BPA. Level of reduced glutathione
also decreased (p < 0.05) in BPA-exposed group. Lower expression of tumor necrosis factor-α (p < 0.05) and interferon-γ
(p < 0.001) was observed in BPA-exposed groups while expression of interleukin-10 increased (p < 0.05) in larvae exposed to
10 μg/l BPA. Moreover, exposure of BPA caused a concentration-dependent increase in expression of heat shock protein 70
(p < 0.05). The present study showed that the exposure to BPA in early life stages of Labeo rohita caused oxidative stress and
suppress NF-κB signaling pathway leading to immunosuppression. The results presented here demonstrate the cross talk between
heat shock protein 70 and cytokines expression.