Research Abstract
Histone post-translational modifications (PTMs) regulate numerous cellular processes, including gene transcription, cell division, and DNA damage repair. Most histone PTMs affect the recruitment or exclusion of reader proteins from chromatin. Here, we present a protocol to measure affinity and interaction kinetics between histone peptides and the recombinant protein using Bio-layer interferometry.
Research Date
Research Department
Research Journal
Bio-protocol
Research Member
Research Vol
8
Research Year
2018
Research Pages
e3012-e3012