Ethanol is one of the most important biofuels that can be produced from different renewable sources. Sugar beet pulp (SBP) is used as renewable and cheap raw material for ethanol production. SBP is the by-product of the sugar industry from sugar beet that is used as animal feed after processing (pressing, dehydration, and pelletizing). Ethanol from SBP will be more profitable value than the other uses as animal feed.The two highest cellulases producer isolates S11 and S88 from the previous work were subjected to DNA identification using the 16S rRNA gene. 16S rRNA is tool used to identify the origin, classification,  evolutionary and relationship history. The isolates S11 (Streptomyces sp. strain FDZH12) and S88 (Streptococcus mitis strain FDZH16) had been submitted to EMBL and their accession numbers are OK033363 and OK033364, respectively. Cellulase gene from S11 Streptomyces FDZH12 then cloned into E.coli to produce superior strain for cellulases production.The recombinant E. coli was confirmed by colony PCR using gene-specific primers of cellulases. Ethanol production from SBP is achieved through three steps: first, acid-base treatment for SBP and then the resulting cellulose content hydrolyzed to fermentable sugar using genetically engineered E.coli cloned by cellulases enzyme. Finally, the fermentable sugar is fermented to ethanol using S.cereviciae FDZH2O The weight of dried SBP after acid-base treatment was 45.5 % of the original dried SBP. Cellulose contents of untreated SBP were 27.95 % and reached 84.22 % after acid-base treatment (842.2g/kg). The maximum yields of glucose by the recombinant E.coli after 24 hours of saccharification of treated SBP were 28.36 g/50 g of acid base treated SBP (67.52% of their cellulose content).