Abstract
This work aimed to study the toxicity of seed neem oil (NO) on oxidative stress in liver, white muscle and blood serum in the fish Oreochromis niloticus and the antioxidant effect of lupine supplementation (LS) against it. Healthy sixty six fish were selected. Two doses of neem oil; 1/20 LC50: 56.3 (NO1) and 1/10 LC50:112.5 PPM (NO2) and three periods of one, two and three weeks were used. The results showed that NO induced marked hyperglycemia and significant elevations in oxidative biomarker total peroxide as well as significant inhibition of antioxidants like catalase and superoxide dismutase activities in most experimental periods in the liver, white muscle and serum. In turn, administration of LS could abolish or at least, improve the adverse effect induced by NO exposure. This study suggests that sublethal doses of NO on fish led to generate oxidative damage in liver and white muscle. In addition, the antioxidant role of lupine seeds may be mainly due to its antihyperglycemic effect, as well as its contents of some natural antioxidants.