n the present work, sulfacetamide (SFA)-modified glassy carbon electrode (MGCE) was successfully constructed by electrochemical modification applying simply cyclic voltammetry (CV) scans. It is well observed that the excellent electrocatalytic ability of SFA film led to much higher oxidation peak current responses compared with bare GCE for epinephrine (EP) oxidation. The EP oxidation takes place at a potential about 120 mV less positive compared with that of an unmodified GCE at the optimum pH 5.5 of phosphate buffer solution (PBS). Applying the anodic stripping differential pulse voltammetry (ASDPV) method and under the optimum conditions, the linear dependence of oxidation peak current on analyte concentrations was found to be (1.0 x10-7-1.5 x10-6) mol L-1, giving detection limit of 2.1 x10-8 mol L-1 and quantification limit of 6.9 x10-8 mol L-1 for EP. The proposed modified electrode exhibited excellent selectivity in the presence of ascorbic acid (AA), glucose (Glu) and uric acid (UA) species whereas dopamine (DA) interfere seriously at higher concentrations. The proposed method showed simplicity, reproducibility, high sensitivity and adequate selectivity. The modified electrode was successfully devoted to EP determination in pharmaceutical formulations and biological fluids; human plasma and urine with acceptable results.