On reviewing literature that focused on the Egyptian spider fauna, it could be concluded that it still incompletely known due to scarcity of studies on this group especially in Upper Egypt. So, the present study was aimed to make a survey of spiders in six different sites covering Assiut governorate, the central part of Egypt, (27º14'N and 31º11'E) and to study its abundance of this group as well as the effects of monthly and seasonally fluctuations on the abundance of the collected taxa. Hand picking method was conducted during day light hours during a period of one year (from December, 2015 till November 2016). Survey results revealed the occurrence of 3457 specimens belonging to 22 families that included 42 genera and 47 species. Family Lycosidae was the most abundant family which represented the highest number during the whole period of study, while family Dictynidae and family Uloboridae were represented by the lowest number in the same period of study. The eudominant species in this survey was Pulchellodromus glaucinus.